mouse anti human igg2 fc pe hp6002 (SouthernBiotech)
SouthernBiotech is a verified supplier 
95
Structured Review
SouthernBiotech
mouse anti human igg2 fc pe hp6002
Mouse Anti Human Igg2 Fc Pe Hp6002, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+human+igg2+fc+pe+hp6002/Mouse+Anti-Human+IgG2+Fc-PE/pmc12842600-11-0-6
Average 95 stars, based on 128 article reviews
Mouse Anti Human Igg2 Fc Pe Hp6002, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 95/100, based on 128 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti+human+igg2+fc+pe+hp6002/Mouse+Anti-Human+IgG2+Fc-PE/pmc12842600-11-0-6
Average 95 stars, based on 128 article reviews
mouse anti human igg2 fc pe hp6002 - by Bioz Stars,
2026-09
95/100 stars
Images
1) Product Images from "ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria"
Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria
Journal: Cell reports
doi: 10.1016/j.celrep.2025.116653
Figure Legend Snippet: IgG reactivity to (A) purified protein derivative (PPD) from Mtb , (B) Mtb cytosolic protein, (C) Mtb culture filtrate, (D) Ag85A and Ag85B, (E) Mtb cell wall, (F) ESAT-6 and CFP-10, and (G) control respiratory syncytial virus (RSV) was determined by customized multiplex Luminex for each individual patient sample with TB over three serial dilutions. Each dot represents relative reactivity for one individual patient sample determined by the area under the curve (AUC), summarizing the median fluorescence intensity (MFI) from serial dilutions. Bars represent median and 95% confidence intervals for latent ( n = 18) and active ( n = 19) TB groups. The p values determined by a Mann-Whitney U test are marked by ^ for significance after adjustment for multiple comparisons by Benjamini-Hochberg.
Techniques Used: Purification, Control, Virus, Multiplex Assay, Luminex, Fluorescence, MANN-WHITNEY
Figure Legend Snippet: (A) Representative chromatograms show patterns of individual glycoforms isolated from the Fc domain of antigen-specific and total bulk IgG in an individual patient with TB, determined by capillary electrophoresis. (B) Violin plots show the relative abundance of sialic acid, galactose, fucose, and bisecting N-acetylglucosamine (GlcNAc) across all individual glycoforms isolated from ESAT-6 and CFP-10 (red) and Mtb cell wall (blue) polyclonal IgG. Each dot represents an individual sample with latent ( n = 18) or active ( n = 19) TB. The median and interquartiles are shown. The dashed lines show the median RSV (green) and total bulk (purple) glycans. The p values determined by a Wilcoxon matched-pairs signed rank test are marked by ^ for significance after adjustment for multiple comparisons by Benjamini-Hochberg.
Techniques Used: Isolation, Electrophoresis
Figure Legend Snippet: (A) Luminescence from the virulent Mtb H37Rv luminescent reporter strain relates to colony-forming units (CFUs). The significance was evaluated by Pearson correlation. (B) To test the effect of antibodies on intracellular Mtb , primary human monocyte-derived macrophages were first infected with the virulent Mtb H37Rv luminescent reporter strain, and the extracellular bacteria were washed away. Then, Mtb -infected primary human monocyte-derived macrophages (MOI = 1) were treated with IgG. Finally, the bacterial burden was quantified with >99% of detectable Mtb in the intracellular as compared to the extracellular medium supernatant compartment. The error bars represent the mean ± SEM. Significance was determined by a Wilcoxon matched-pairs signed rank test. (C) Daily Mtb luminescence measurements representing the median of endemic control, active TB, and latent TB samples are shown for one representative healthy donor of human macrophages. (D) Data from n = 3 healthy human macrophage donors in independent experiments are summarized, with each dot representing the Mtb burden for each individual patient with TB relative to control polyclonal IgG. The median and 95% confidence interval (CI) are shown. The dashed line shows the median of endemic IGRA− control individuals. The significance was determined by a Mann-Whitney U test.
Techniques Used: Derivative Assay, Infection, Bacteria, Control, MANN-WHITNEY
Figure Legend Snippet: (A) The relationships between ESAT-6 and CFP-10 IgG levels and subclasses and intracellular Mtb burden within individuals with latent and active TB were evaluated by Spearman correlation. Heatmaps depict the Spearman rank correlation coefficient, ** p ≤ 0.01, and ^ stands for significance after adjustment for multiple comparisons by Benjamini-Hochberg. The scatterplot shows ESAT-6 and CFP-10 IgG1, and the Mtb burden is shown in the scatterplot, with each dot representing each individual with latent TB. (B) As a control, the relationships between Mtb cell-wall IgG levels and subclasses and intracellular Mtb burden are shown.
Techniques Used: Control
Figure Legend Snippet: (A) Each column in the histogram depicts the intracellular Mtb burden of one individual patient with latent (light gray) and active (dark gray) TB as in . The dashed line represents the intracellular Mtb burden with control IgG. (A and B) The anti- Mtb activity of IgG from each individual patient with TB was determined by the difference in Mtb burden between control and patient IgG (red). (C) For the n = 17 latent and n = 14 active TB samples with detectable anti- Mtb activities relative to ESAT-6 and CFP-10 IgG1, the relationships to ESAT-6 and CFP-10 IgG Fc glycans as determined by Spearman correlations are listed with ^ marking the significance after adjustment for multiple comparisons by Benjamini-Hochberg. (D) N-glycans from anti-ESAT-6 and CFP-10 mAb were enzymatically removed with PNGase F and then used to treat Mtb -infected primary human monocyte-derived macrophages. The intracellular Mtb burden is shown relative to a no-antibody (Ab) control. The graph summarizes data for n = 6 healthy macrophage donors, with each line representing a single donor. (E) An L234A and L235A (LALA) variant of anti-ESAT-6 and CFP-10 mAb was used to treat Mtb -infected primary human monocyte-derived macrophages. Each line represents a single healthy macrophage donor ( n = 9). The significance was determined by a Wilcoxon matched-pairs signed rank test.
Techniques Used: Control, Activity Assay, Infection, Derivative Assay, Variant Assay
Related Articles
Purification:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Control:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Virus:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Multiplex Assay:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Luminex:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Fluorescence:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: MANN-WHITNEY:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Isolation:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Electrophoresis:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Derivative Assay:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Infection:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Bacteria:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Activity Assay:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Variant Assay:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Binding Assay:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Glycoproteomics:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Two Tailed Test:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Clinical Proteomics:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Recombinant:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Magnetic Beads:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Cell Stimulation:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Staining:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Electron Microscopy:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Sequencing:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Modification:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Luciferase:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Liquid Chromatography:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Mass Spectrometry:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Software:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Sterility:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: Suspension:Article Title: Afucosylation of HLA-specific IgG1 as a potential predictor of antibody pathogenicity in kidney transplantation Article Snippet: Article Title: ESAT-6 and CFP-10 reactive IgG in patients with tuberculosis inhibits intracellular bacteria Article Snippet: |